Evaluation of Some Promising Teosinte Genotypes for Morphological and Genetic Parameters under Egyptian Conditions
Hend E. Habiba , Abd El Aziz. T. Bondok and Mohmed. S. Abdel-Aal
This investigation was conducted at Gemmeiza, Agric. Res. Station, El –Gharbia Governorate, Agricultural Research Center (ARC), Egypt, during the period between 2014 and 2015 summer seasons to evaluate eight selected teosinte genotypes. The experimental design was a randomized complete block with three replicates. The data measured were fresh yield (fr) and dry yield (dy) over the consecutive cuts. Plant height (ph), stem dimeter (SD), number of leaves on the main stem (NL), leaf area per plant, dry matter (DM), fresh yield, dry yield, fresh leaves / stem (%) and dry leaves / stem (%) and were recorded at cutting time.
Heritability estimate, in the broad – sense (h2b), on mean basis for traits, were carried out. Genotypes 3 and 4 were the superior to the other tested genotypes in morphological characters in both seasons. The heritability was high in fresh yield (fy) (99.9%) and dry matter (DM) (99.8%), followed by plant height (99.5%) and leaf area (99.5%).
(SDS – PAGE) official sodium dodecyl sulphate polyacrylamide gel electrophoresis was employed to detect variation in total soluble protein content technique among the eight genotypes of teosinte; namely, 1, 2, 3, 4, , 6, 7 and 8 genotypes. Different protein fragmentation was recorded for the teosinte genotypes, which ranged from 21 to 16 for 1, 2, 3 and 4 genotypes repectively, with molecular weight (MW) ranged from 154 to 7 kda. It was found that similarity and dissimilarity, for protein patterns of teosinte genotypes, showed that 3 and 4 genotypes showed the same protein patterns, with almost protein loci and molecular weights, with 18 and 16 protein bands. According to electrophoretic study. Phyllogenetic tree was constructed and indicated a clear genetic base from SDS – PAGE analysis, high similarity percentage i.e. (94.5%) for genotype 3, and the lowest similarity percentage (76.2%) for the fist genotype.
The genotypes were divided into three main groups; i.e., the genotypes (1, 8), were in one group, while genotype (2) was in the second group and (3, 4, 5, 6 and 7) genotypes were in third group.
Key words : Teosinte, genotypic, evaluation, SDS – PAGE and heritability in broad sense.