Modulation of Abamectin and Indoxacarb -Induced Toxicity on Male Albino Rats by Moringa oleifera

Mona A. Abdelrasoul

Pesticide formulations are characterized by its complex mixtures.The available toxicity information on the active ingredients alone is not quit enough to evaluate the risk of undesirable health effects of commercial pesticides. Therefore, the present study was proposed to clarify the adverse effects of exposure to formulated abamectin (ABA) (0.5 mg/kg bw, 1/20 LD50), indoxacarb (IND) (86.6 mg/kg bw, 1/20 LD50 ) and their combinations on some haematological and biochemical parameters and histopathological changes of male rats given repetitive oral doses for 30 consecutive days. The protective effect of aqueous extract of Moringa oleifera leaves (MO) against toxicity induced by tested insecticide was, also, considered.  Results indicated that ABA, IND, and their combination treatments, significantly, caused gradual decreasing in body weight, erythrocyte counts (RBCs), haemoglobin concentration (g/dL), haematocrit (%) and platelets (PLT). However, significant elevation in the relative liver, kidney and brain weights were associated with these treatments. Marked and significant increments in the levels of serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (γ-GT), glucose  concentration  and creatinine (Cre), while decrease in serum acetylcholinesterase (AChE) activity  was observed due to tested insecticide treatments. Exposure to ABA and IND, significantly decrease the total protein and total antioxidant scavenging activity. Histological studies confirmed the biochemical parameters. M. oleifera leaves-treated rats with ABA, IND or their combinations showed marked improvements of hematological, biochemical parameters and histopathological changes, indicating  that MO extract bear a potent antioxidant activity. Therefore, the successive aqueous extract of MO as an herbal tea may be beneficial for patients who suffer from liver, kidney and brain diseases and restoring the damage sustained by insecticide exposure.


Keywords:Rats, Abamectin, Indoxacarb; biomarkers, Histopathology, Moringa, modulation