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In Vitro Propagation and Somatic Embryogenesis of Marvel of Peru (Mirabilis jalapa L.) from Nodal and Leaf Explants

Mohamed K.Gaber

Nowadays, there is a tremendous demands for Mirabilis jalapa L., it is a popular ornamental landscape plant grown worldwide for the beauty of its flowers, also earned its place in herbal medicine practices around the world for traditional medicine by the people from different countries. Therefore, in vitro propagation and somatic embryogenesis of Mirabilis jalapa was achieved during both seasons of 2012 and 2013, from nodal and leaf explants on MS medium supplemented with different concentrations and combinations of NAA, KIN, IBA and 2,4-D. Respecting in vitro propagation, the highest mean number of shoots (2.67) formed per propagule were recorded on MS medium supplemented with 3.0 mg/l KIN and 0.250 mg/l NAA. Regarding to rhizogenesis stage, it is obvious that, fortifying MS-medium with a combination of both IBA at 0.00 mg/l, and NAA at 0.500 mg/l, gave the highest mean value of roots formed per propagule (6.11). On the other end, the best results of embryonic callus induction response (100%) and embrogenic callus size per propagule (++++) in whole area of leaf explant was observed on MS medium augmented with 4.0 mg/l 2,4-D. Neoformed plantlets were successfully acclimated and established in peat and sand in ratio of 1:1, and flowered successfully under field conditions